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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in lots of tumors, which leads to chromosome instability, and thus may contribute to tumorigenesis. This protein was observed to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important function in marketing mobile-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are described. Two added splice variants were being claimed but detailed nucleotide sequence details is not really nonetheless obtainable.
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Blocking peptides are peptides that bind specially to your goal antibody and block antibody binding. These peptide generally contains the epitope identified because of the antibody.
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This guidebook outlines even further probable optimization of mobile and immunoassay parameters to make certain the best possible effects are...
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This guide outlines further probable optimization of cellular and immunoassay parameters to be sure the best possible success are...
Antibodies bound to the blocking peptide no more bind to the epitope within the goal protein. By evaluating the staining from the blocked antibody versus the antibody by yourself, one can see which staining is specific.
Focus on Data Cyclin E belongs for the really conserved cyclin loved ones, whose customers are characterized by a remarkable periodicity in protein abundance with the cell cycle. Cyclins function as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation patterns which lead to the temporal coordination of each and every mitotic event. Cyclin E varieties a complex with and functions as being a regulatory subunit of CDK2, whose action is required for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is also degraded as cells progress by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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